The PYthon Microscopy Environment is an open-source package providing image acquisition and data analysis functionality for a number of microscopy applications, but with a particular emphasis on single molecule localisation microscopy (PALM/STORM/PAINT etc ...). The package is multi platform, running on Windows, Linux, and OSX.
PYME offers a data acquisition module which performs the microscope and camera control functionality similar to that offered by e.g. micro-manager, but optimised for PALM/STORM type imaging. Some features include:
The real time analysis component allows analysis to be performed in real time, distributing the load across a small cluster if required.
Offers a fast and flexible tool for exploring and visualising the list of fluorophore locations that is produced by PALM/STORM analysis algorithms.
A slice based image viewer for 2D, 3D, and 4D data sets, supporting many basic image processing tasks.