PYME.Analysis.PSFGen.ps_app module

Gibson & Lanni PSF approximation

PYME.Analysis.PSFGen.ps_app.genWidefieldPSF()

Generate a (shifted, spherically abberated) widefield PSF based on the paraxial approximation. Arguments are: ‘X’, ‘Y’, ‘Z’, ‘P’ , ‘A’=1,’x0’=0, ‘y0’=0, ‘z0’=0, ‘k’=2*pi/488, ‘NA’=1.3, ‘depthInSample’=20um, ‘nImmersionCorr’=1.5, ‘nSample’=1.47,’nCoverslipCorr’=1.5, ‘nImmersionSample’=1.5, ‘nCoverslipSample’=1.5, ‘CoverslipThicknessCorr’=175um, ‘CoverslipThicknessSample’=175um, ‘ImmersionThicknessSample’=90um. All values should be given in nm.

PYME.Analysis.PSFGen.ps_app.genWidefieldPSFW()

Generate a (shifted, spherically abberated) widefield PSF based on the paraxial approximation. Arguments are: ‘X’, ‘Y’, ‘Z’, ‘P’, ‘W’ , ‘A’=1,’x0’=0, ‘y0’=0, ‘z0’=0, ‘k’=2*pi/488, ‘NA’=1.3, ‘depthInSample’=20um, ‘nImmersionCorr’=1.5, ‘nSample’=1.47,’nCoverslipCorr’=1.5, ‘nImmersionSample’=1.5, ‘nCoverslipSample’=1.5, ‘CoverslipThicknessCorr’=175um, ‘CoverslipThicknessSample’=175um, ‘ImmersionThicknessSample’=90um. All values should be given in nm.