Modifying PYMEAcquire and writing hardware drivers

Initial setup

  • Download and install a development build following the instructions at http://python-microscopy.org/doc/Installation/InstallationFromSource.html

  • Run PYMEAcquire (either from the console, or by making a shortcut to it). When run without parameters, it uses PYME/Acquire/Scripts/init.py as a startup script and should run with a simulated camera. Copy and rename this script keeping it in the Acquire/Scripts directory (this will be your new hardware config script, and you can slowly edit it to use real hardware rather than the simulated bits) [NB - support for init scripts in other directories is on our wishlist]. You can specify which initialization script to use with the -i command line option to PYMEAcquire

Making a camera driver

This can be a little involved

  • Subclass PYME.Acquire.Hardware.Camera.Camera and implement the methods for your camera. It will probably be hepful to refer the drivers of other similar cameras when doing this. Note that for most sCMOS cameras it is the drivers responsibility to handle circular buffers and the like. The Zyla code might be a good place to go for inspiration here.

  • [optional] In the same file as your new camera, also add a class which inherits from both PYME.Acquire.Hardware.Camera.MultiviewMixIn.

  • change your init_XXX.py script to initialize your new camera class instead of the fake camera. Register the camera with the microscope using scope.register_camera().

Making a driver for a stage / piezo

Copy one of the existing piezo drivers (in PYME/Acquire/Hardware/Piezos) and modifiy the method implementation whilst keeping the signatures the same. Should really be re-factored to use a common Piezo/Positioning base class. Both piezos and motorized stages use the same interface.

Making a driver for a laser

This one actually has a base class! Copy one of the existing laser drivers and modify accordingly.

Other plugins / general comments

Other hardware doesn’t currently have a defined interface, so there is a fair bit of flexibility about how something can be implemented. See filter wheel code for an example.

In general we are moving from an imperative (we tell the piezo to move to a position x) to a state based (we tell the piezo that it should be at position x) way of talking to hardware. This is to aid future automation (we can save and restore the ‘state’ of the microscope / tell the microscope to assume a certain state, rather than making a number of imperative commands to each of the hardware components). This is still a work in progress, but new hardware should ideally support this way of doing things - see PYME.Acquire.microscope, and in particular the PYME.Acquire.microscope.StateHandler code for more info.

Timing

Timing is currently ‘lazy’ by default - i.e. there is not tight synchronization between hardware movements and the camera, with the preferred approach being to generate and save a timestamped event (PYME.Acquire.eventLog - although this still needs documentation) for any hardware motion and compare this to frame timestamps in post-processing (currently inferred from a start time and frame rate, but ideally provided directly from the camera in the future - both Andor and Hamamatsu cameras support hardware timestamps, although these are not supported in our current software). The rationale behind the asynchronous acquisition is to allow the maximum frame rate possible. That said, nothing about our design prevents you from slaving other hardware off the camera trigger, and triggering the camera externally should be possible with minor modifications to the camera drivers.

Using the state based control, it is also possible to force camera synchronization at a software level, albeit at a significant loss of speed (synchronized operations involve stopping and restarting the camera).